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Immunoassay using ion selective electrode and protein pendant liposomes
http://hdl.handle.net/10252/1096
http://hdl.handle.net/10252/1096377992d6-ddc2-4c1f-8c00-84741e90813d
名前 / ファイル | ライセンス | アクション |
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9_jnlpdf.pdf (279.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-09-10 | |||||
タイトル | ||||||
タイトル | Immunoassay using ion selective electrode and protein pendant liposomes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ion channel | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunosensor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biosensor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | thin-layer potentiometry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human IgG | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Abe, Hirohisa
× Abe, Hirohisa× Kataoka, Masamitsu× Yasuda, Tatsuji× Umezawa, Yoshio |
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書誌情報 |
Analytical sciences 巻 2, 号 6, p. 523-527, 発行日 1986-06 |
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出版者 | ||||||
出版者 | 日本分析化学会 | |||||
ISSN / EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0910-6340 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.2.523 | |||||
書誌ID(NCID) | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
テキストバージョン | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 433 | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 化学 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Immunoassay for an anti-human IgG antibody and a human IgG is presented. Thin-layer potentiometry, i.e., a combination of fluoride ion selective electrode and silver chloride coated silver-plate reference electrode was used to monitor fluoride release from the multilamellar liposome. Human IgG was attached to the surface of liposome membrane by the use of cross-linking reagent N-hydroxysuccinimidyl3-(2-pyridyldithio)propionate (SPDP). The antigen/antibody/complement reaction triggered the formation of "channel-like" holes across the liposome membrane which enabled entrapped fluoride anion flow through the hole. The fluoride ion release was specific for anti-human lgG and depended on the presence of complement. By the use of human IgG pendant liposomes, determination of about 10^-14 mol of anti-human IgG (rabbit) and 10^-14 mol human IgG was feasible. |