@article{oai:barrel.repo.nii.ac.jp:00000914,
 author = {Abe, Hirohisa and Kataoka, Masamitsu and Yasuda, Tatsuji and Umezawa, Yoshio},
 issue = {6},
 journal = {Analytical sciences},
 month = {Jun},
 note = {Immunoassay for an anti-human IgG antibody and a human IgG is presented. Thin-layer potentiometry, i.e., a combination of fluoride ion selective electrode and silver chloride coated silver-plate reference electrode was used to monitor fluoride release from the multilamellar liposome. Human IgG was attached to the surface of liposome membrane by the use of cross-linking reagent N-hydroxysuccinimidyl3-(2-pyridyldithio)propionate (SPDP). The antigen/antibody/complement reaction triggered the formation of "channel-like" holes across the liposome membrane which enabled entrapped fluoride anion flow through the hole. The fluoride ion release was specific for anti-human lgG and depended on the presence of complement. By the use of human IgG pendant liposomes, determination of about 10^-14 mol of anti-human IgG (rabbit) and 10^-14 mol human IgG was feasible.},
 pages = {523--527},
 title = {Immunoassay using ion selective electrode and protein pendant liposomes},
 volume = {2},
 year = {1986}
}